SEC. 565. None of the funds made available by this or any other Act may be used to investigate, litigate, or advocate against any person or recipient, as currently defined at section 106.2 of title 34, Code of Federal Regulations, for defining ‘‘sex’’ as currently used in, inter alia, section 1681 of title 20, United States Code, and sections 106.21, 22, 23, 30, 31, 32, 33, 34, 35, 37, 39, 40, 41, 44, and 45 of title 34, Code of Federal Regulations, to mean biological sex, male or female, as determined by the type of gamete an individual produces; and for defining "boys and girls" to mean only biological boys, whose DNA consists of one X sex chromosome and one Y sex chromosome, and biological girls, whose DNA consists of two X sex chromosomes.

SEC. 581. None of the funds made available by this Act may be used to sue any State or local government over any law protecting single sex sports, single sex facilities, or limiting transgender medical procedures, including in insurance coverage and age limitations, or to intervene or file an amicus brief in such a case.

SEC. 606. None of the funds made available by this Act may be used by the Bureau of Prisons to assign an individual to a facility that does not correspond to such individual's biological sex.